Which of the following represents the use of osmotic pressure as a microbial control method?
A. bleaching a kitchen counter
B. salting meat
C. rinsing...
Antimicrobial agents can target the cell wall by
Antimicrobial agents can target the cell wall by
A. blocking its synthesis.
B. digesting it.
C. inhibiting peptidoglycan synthesis.
D. All of the...
In lab, inoculating loops are sterilized using
In lab, inoculating loops are sterilized using
A. moist heat.
B. chemicals.
C. incineration.
D. filtration.
E. ethylene oxide.
Answer:...
Which of the following acids is not used to destroy or inhibit microbial cells in food?
Which of the following acids is not used to destroy or inhibit microbial cells in food?
A. acetic acid
B. benzoic acid
C. lactic acid
D. phosphoric...
Ethylene oxide is
Ethylene oxide is
A. sporicidal.
B. only effective with high heat.
C. the active agent in household bleach.
D. used as an antiseptic against anaerobes.
E....
Which of the following is not used as an antiseptic?
Which of the following is not used as an antiseptic?
A. iodophor
B. chlorhexidine
C. 3% hydrogen peroxide
D. betadine (povidone-iodine)
E. glutaraldehyde
Answer:...
Heavy metals work by
Heavy metals work by
A. rupturing the cell membrane.
B. inactivating proteins.
C. binding to DNA.
D. dissolving the cell wall.
E. mutating DNA.
Answer:...
All of the following disrupt the cytoplasmic membrane except
All of the following disrupt the cytoplasmic membrane except
A. detergents.
B. quaternary ammonium compounds.
C. high concentration phenols.
D. iodine.
Answer:...
Hydrogen peroxide can be
Hydrogen peroxide can be
A. sporicidal.
B. fungicidal.
C. bactericidal.
D. virucidal.
E. All of the choices are correct.
Answer: ...
The chemical agent that produces highly toxic and reactive free radicals is
The chemical agent that produces highly toxic and reactive free radicals is
A. cidex.
B. cationic detergents.
C. hydrogen peroxide.
D. chlorhexidine.
E....
Alcohols
Alcohols
A. dissolve membrane lipids at concentrations of greater than 50%.
B. can be used for disinfection or antisepsis.
C. are skin degerming...
The compound that is an organic base containing chlorine and two phenolic rings, and is used increasingly for mucous membrane irrigation, obstetrical washes, hand scrubbing, and prepping surgical skin sites is
The compound that is an organic base containing chlorine and two phenolic rings, and is used increasingly for mucous membrane irrigation, obstetrical...
____ is a halogen used in gaseous and liquid form for large-scale disinfection of drinking water and sewage.
____ is a halogen used in gaseous and liquid form for large-scale disinfection of drinking water and sewage.
A. Iodine
B. Chlorine
C. Bromine
D....
All of the following pertain to hypochlorites except
All of the following pertain to hypochlorites except
A. release hypochlorous acid in solution.
B. cause denaturation of enzymes.
C. found in iodophors.
D....
Which of the following is not a factor that affects germicidal activity?
Which of the following is not a factor that affects germicidal activity?
A. the material being treated
B. the length of exposure
C. the strength of...
_____ is a control method that removes microorganisms rather than inhibiting or killing them.
_____ is a control method that removes microorganisms rather than inhibiting or killing them.
A. Boiling
B. Sterilization
C. Radiation
D. Filtration
E....
Filtration can result in sterilization of serum and blood products
Filtration can result in sterilization of serum and blood products
A. by removing viruses.
B. by relying on gravity.
C. by removing toxins.
D. using...
HEPA filters are used to remove microbes from
HEPA filters are used to remove microbes from
A. air.
B. liquids.
C. human tissues.
D. medical instruments.
E. All of the choices are correct.
Answer:...
Which of the following forms of radiation is in order from the most penetrating to the least penetrating?
Which of the following forms of radiation is in order from the most penetrating to the least penetrating?
A. gamma, cathode, X rays
B. gamma, X rays,...
Which control method would not be a suitable choice for killing Mycobacterium in a capped culture tube?
Which control method would not be a suitable choice for killing Mycobacterium in a capped culture tube?
A. ultraviolet (germicidal) light
B. gamma...
Which of the following items are typically irradiated in order to kill microbes?
Which of the following items are typically irradiated in order to kill microbes?
A. meats like ground beef and pork
B. human tissues such as heart...
_____ radiation excites atoms to a higher energy state within molecules such as DNA that then leads to the formation of pyrimidine dimers.
_____ radiation excites atoms to a higher energy state within molecules such as DNA that then leads to the formation of pyrimidine dimers.
A. Infrared
B....
Removal of moisture by dehydration is called
Removal of moisture by dehydration is called
A. desiccation.
B. flash freeze.
C. lyophilization.
D. pasteurization.
E. sterilization.
Answer:...
Vials of microorganisms that undergo the freeze-drying process called _____ will remain preserved and viable for years.
Vials of microorganisms that undergo the freeze-drying process called _____ will remain preserved and viable for years.
A. desiccation
B. flash freeze
C....
What instrument is most effective for pressure-temperature sterilization?
What instrument is most effective for pressure-temperature sterilization?
A. oven
B. autoclave
C. water bath
D. Bunsen burner
E. incubator
Answer:...
A method for sterilizing milk, called _____ treatment, uses 134C for 1 to 2 seconds.
A method for sterilizing milk, called _____ treatment, uses 134C for 1 to 2 seconds.
A. pasteurization
B. batch pasteurization
C. flash pasteurization
D....
_____ heat is more rapidly effective and efficient compared to _____ heat.
_____ heat is more rapidly effective and efficient compared to _____ heat.
A. High, dry
B. High, moist
C. Dry, moist
D. Moist, dry
E. Moist, high
Answer:...
Pasteurization
Pasteurization
A. kills all vegetative forms.
B. reduces the number of vegetative forms.
C. reduces the number of endospores.
D. increases food nutrient...
Placing organisms at 4 C is
Placing organisms at 4 C is
A. bactericidal.
B. bacteriostatic.
C. decontamination.
D. sterilization.
E. None of the choices is correct.
Answer:...
Disinfection of beverages such as apple juice, milk, wine is optimally achieved by
Disinfection of beverages such as apple juice, milk, wine is optimally achieved by
A. pasteurization.
B. chlorination.
C. moist heat autoclave.
D....
The lowest temperature needed to kill all microbes in 10 minutes is the
The lowest temperature needed to kill all microbes in 10 minutes is the
A. thermal death point (TDP).
B. thermal death time (TDT).
C. sporicidal...
The shortest time required to kill all the microbes in a sample at a specified temperature is called the
The shortest time required to kill all the microbes in a sample at a specified temperature is called the
A. thermal death point (TDP).
B. thermal...
The most efficient sterilizing conditions in a steam autoclave are
The most efficient sterilizing conditions in a steam autoclave are
A. 121C at 15 psi for 15 minutes.
B. 63C for 30 minutes.
C. 160C for 2 hours.
D....
Dry heat
Dry heat
A. is less efficient than moist heat.
B. cannot sterilize.
C. includes tyndallization.
D. is used in devices called autoclaves.
E. will sterilize...
Sterilization is achieved by
Sterilization is achieved by
A. flash pasteurization.
B. hot water.
C. boiling water.
D. steam autoclave.
E. All of the choices are correct.
Answer:...
Physical agents for controlling microbial growth include all the following except
Physical agents for controlling microbial growth include all the following except
A. ultraviolet radiation.
B. boiling water.
C. HEPA filters.
D....
Which of the following does not affect microbial nucleic acids?
Which of the following does not affect microbial nucleic acids?
A. moist heat
B. ultraviolet light
C. X rays
D. ethylene dioxide
E. formaldehyde
Answer:...
Agents that can denature microbial proteins include all of the following except
Agents that can denature microbial proteins include all of the following except
A. moist heat.
B. alcohol.
C. acids.
D. metallic ions.
E. X rays.
Answer:...
Some microbial control agents are able to _____ cell proteins by breaking bonds that maintain the native state (three-dimensional configuration) of the proteins.
Some microbial control agents are able to _____ cell proteins by breaking bonds that maintain the native state (three-dimensional configuration) of...
Surfactants work by
Surfactants work by
A. coating the organism, preventing interaction with its environment.
B. blocking transport into the organism.
C. blocking transport...
Each of the following is the target of antimicrobial agents except
Each of the following is the target of antimicrobial agents except
A. cell walls.
B. cell membranes.
C. ribosomes.
D. cellular proteins.
E. cytoplasm.
Answer:...
Microbial death occurs when there is
Microbial death occurs when there is
A. no movement.
B. permanent loss of reproduction.
C. a change in appearance.
D. a decrease in size.
E. All of...
Which of the following factors will influence the action of microbial agents?
Which of the following factors will influence the action of microbial agents?
A. the number of microorganisms
B. the kind of microorganisms
C. temperature...
Which is correct regarding the rate of microbial death?
Which is correct regarding the rate of microbial death?
A. Cells die at increasingly greater rates.
B. Only older cells die in a culture.
C. Cells...
A cleansing method that mechanically removes microbes and other debris to reduce contamination is
A cleansing method that mechanically removes microbes and other debris to reduce contamination is
A. disinfection.
B. sterilization.
C. antisepsis.
D....
The alcohol wipe before an injection is an example of
The alcohol wipe before an injection is an example of
A. antisepsis.
B. disinfection.
C. sterilization.
D. decontamination.
E. virilization.
Answer:...
The betadine swab before blood donation is an example of
The betadine swab before blood donation is an example of
A. antisepsis.
B. disinfection.
C. sterilization.
D. decontamination.
E. sanitation.
Answer:...
The removal of all life forms from inanimate objects is termed
The removal of all life forms from inanimate objects is termed
A. antisepsis.
B. disinfection.
C. sterilization.
D. decontamination.
E. degerming.
Answer:...
The method of removing vegetative life forms from living surfaces is termed
The method of removing vegetative life forms from living surfaces is termed
A. antisepsis.
B. disinfection.
C. sterilization.
D. decontamination.
E....
The method of removing vegetative microbial life forms from inanimate objects is termed
The method of removing vegetative microbial life forms from inanimate objects is termed
A. antisepsis.
B. disinfection.
C. sterilization.
D. decontamination.
E....
The easiest microbial forms to kill or inhibit are
The easiest microbial forms to kill or inhibit are
A. prions.
B. vegetative bacteria and fungi.
C. endospores.
D. protozoan cysts.
E. mycobacteria...
Which of the following types of control agents would be used to achieve sterility?
Which of the following types of control agents would be used to achieve sterility?
A. virucide
B. bactericide
C. germicide
D. sporicide
E. fungicide
Answer:...
Scrubbing or immersing the skin in chemicals to reduce the numbers of microbes on the skin is
Scrubbing or immersing the skin in chemicals to reduce the numbers of microbes on the skin is
A. disinfection.
B. sterilization.
C. antisepsis.
D....
The use of chemical agents directly on exposed body surfaces to destroy or inhibit vegetative pathogens is
The use of chemical agents directly on exposed body surfaces to destroy or inhibit vegetative pathogens is
A. disinfection.
B. sterilization.
C....
The use of a physical or chemical process to destroy vegetative pathogens is
The use of a physical or chemical process to destroy vegetative pathogens is
A. disinfection.
B. sterilization.
C. antisepsis.
D. sanitization.
E....
The process of using a cleansing technique to mechanically remove and reduce microorganisms and debris to safe levels is
The process of using a cleansing technique to mechanically remove and reduce microorganisms and debris to safe levels is
A. disinfection.
B. sterilization.
C....
The process that destroys or removes all microorganisms and microbial forms including bacterial endospores on inanimate objects is
The process that destroys or removes all microorganisms and microbial forms including bacterial endospores on inanimate objects is
A. disinfection.
B....
Which of the following microbial forms have the highest resistance to physical and chemical controls?
Which of the following microbial forms have the highest resistance to physical and chemical controls?
A. naked viruses
B. protozoan cysts
C. fungal...
_____ is a mechanism of release for enveloped viruses.
_____ is a mechanism of release for enveloped viruses.
A) Lytic replication
B) Persistent infection
C) Oncogenesis
D) Latency
E) Budding
Answer:...
Virus infection results in cancer in the process of
Virus infection results in cancer in the process of
A) lytic replication.
B) budding.
C) oncogenesis.
D) persistent infection.
E) latency.
Answer:...
Virus replication results in the death of the cell in a(n) __________ infection.
Virus replication results in the death of the cell in a(n) __________ infection.
A) lytic
B) latent
C) persistent
D) oncogenic
E) both latent and...
During __________, viruses remain dormant in a cell.
During __________, viruses remain dormant in a cell.
A) budding
B) latency
C) lytic replication
D) persistent infection
E) oncogenesis
Answer:...
Viruses are shed slowly and steadily during
Viruses are shed slowly and steadily during
A) lytic replication.
B) budding.
C) oncogenesis.
D) persistent infection.
E) latency.
Answer:...
Proteins are present in
Proteins are present in
A) cells only.
B) viruses only.
C) viroids only.
D) both cells and viruses.
E) cells, viruses, and viroids.
Answer:...
Cytoplasm is a characteristic of
Cytoplasm is a characteristic of
A) cells only.
B) viruses only.
C) both cells and viruses.
D) viroids only.
E) both viroids and viruses.
Answer:...
Double-stranded RNA genomes can be found
Double-stranded RNA genomes can be found
A) only in cells.
B) in both cells and viruses.
C) only in viruses.
D) in prions.
E) in both prions and...
Double-stranded DNA genomes are found
Double-stranded DNA genomes are found
A) only in viroids.
B) only in cells.
C) only in viruses.
D) in both cells and viruses.
E) in both viruses...
A lipid membrane is present
A lipid membrane is present
A) only in viruses.
B) in both cells and viruses.
C) only in cells.
D) only in prions.
E) in both prions and viroids.
Answer:...
The infectious particles of fungi have RNA genomes and lack a capsid. They are therefore similar to
The infectious particles of fungi have RNA genomes and lack a capsid. They are therefore similar to
A) bacteriophages.
B) enveloped viruses.
C) prions.
D)...
How are prions different from all other known infectious agents?
How are prions different from all other known infectious agents?
A) They cannot reproduce outside a cell.
B) They act as slow viruses.
C) They cause...
Viroids infect
Viroids infect
A) bacteria.
B) plants.
C) fungi.
D) plants and animals.
E) all organisms.
Answer:...
Diploid cell cultures and continuous cell cultures differ in which of the following ways?
Diploid cell cultures and continuous cell cultures differ in which of the following ways?
A) longevity
B) source of cells
C) convenience of use
D)...
Plaque assays are used for
Plaque assays are used for
A) evaluating the cancer-causing ability of a virus.
B) estimating the number of phages in a culture.
C) the study of...
Tumors invade other organs and tissues in a process called
Tumors invade other organs and tissues in a process called
A) neoplasia.
B) a plaque assay.
C) latency.
D) budding.
E) metastasis.
Answer:...
One mechanism by which viruses may cause cancer is to interrupt the genetic regulatory sequences of repressor proteins. Which of the following types of viruses is most likely to be involved in causing cancer by this mechanism?
One mechanism by which viruses may cause cancer is to interrupt the genetic regulatory sequences of repressor proteins. Which of the following types...
Which of the following individuals discovered prions?
Which of the following individuals discovered prions?
A) Lacks
B) Iwanowsky
C) Prusiner
D) Stanley
E) Rous
Answer:...
How is the HIV provirus different from a lambda phage prophage?
How is the HIV provirus different from a lambda phage prophage?
A) The HIV provirus is integrated permanently into the host cell's DNA.
B) All subsequent...
The majority of cases of infant diarrhea are caused by what kind of virus?
The majority of cases of infant diarrhea are caused by what kind of virus?
A) dsDNA viruses
B) dsRNA viruses
C) +ssRNA viruses
D) retroviruses
E)...
Which of the following membranes can give rise to a viral envelope?
Which of the following membranes can give rise to a viral envelope?
A) the nuclear membrane
B) the cytoplasmic membrane
C) the endoplasmic reticulum
D)...
In contrast to most dsDNA animal viruses, the poxviruses replicate solely in the cytoplasm of the host cell. This fact implies that the viral genome may encode
In contrast to most dsDNA animal viruses, the poxviruses replicate solely in the cytoplasm of the host cell. This fact implies that the viral genome...
Which of the following types of animal virus requires RNA-dependent RNA transcriptase to be replicated?
Which of the following types of animal virus requires RNA-dependent RNA transcriptase to be replicated?
A) +ssRNA viruses
B) ssDNA viruses
C) -ssRNA...
The genome of which of the following types of animal virus can act directly as mRNA?
The genome of which of the following types of animal virus can act directly as mRNA?
A) retroviruses
B) +ssRNA viruses
C) -ssRNA viruses
D) ssDNA...
Reverse transcriptase is associated with which of the following?
Reverse transcriptase is associated with which of the following?
A) dsDNA viruses
B) +ssRNA viruses
C) -ssRNA viruses
D) retroviruses
E) dsRNA viruses
Answer:...
Which of the following is matched INCORRECTLY?
Which of the following is matched INCORRECTLY?
A) poliovirus — direct penetration
B) herpesvirus — endocytosis
C) adenovirus — membrane fusion
D)...
Zones of clearing in cell cultures that are the result of virus infection are called plaques. Sometimes "cloudy plaques" are seen on bacterial cultures infected with bacteriophage. What type of viral infection might cause this appearance?
Zones of clearing in cell cultures that are the result of virus infection are called plaques. Sometimes "cloudy plaques" are seen on bacterial cultures...
Which of the following agents is capable of inducing conversion of a prophage back to a lytic phage?
Which of the following agents is capable of inducing conversion of a prophage back to a lytic phage?
A) UV light
B) X rays
C) presence of +ssRNA
D)...
Why is lysogeny advantageous to a bacteriophage?
Why is lysogeny advantageous to a bacteriophage?
A) It speeds up the viral infection cycle.
B) The genetic material of the bacteriophage can be...
Which of the following events occurs in the lytic cycle of bacteriophage T4 infection but NOT in the lysogenic cycle?
Which of the following events occurs in the lytic cycle of bacteriophage T4 infection but NOT in the lysogenic cycle?
A) attachment
B) entry
C) digestion...
The phenomenon of transduction is associated with which of the stages of a bacteriophage infection cycle?
The phenomenon of transduction is associated with which of the stages of a bacteriophage infection cycle?
A) attachment
B) assembly
C) release
D)...
The enzyme lysozyme is critical for which of the stages of a bacteriophage T4 infection cycle?
The enzyme lysozyme is critical for which of the stages of a bacteriophage T4 infection cycle?
A) entry
B) assembly
C) entry and release
D) synthesis
E)...
Which of the following is associated with the attachment of a bacteriophage to a bacterial cell?
Which of the following is associated with the attachment of a bacteriophage to a bacterial cell?
A) random collisions
B) chemical attractions
C) receptor...
Put the following stages of a lytic replication cycle in order, from earliest to latest stages:
Put the following stages of a lytic replication cycle in order, from earliest to latest stages:
I. Synthesis
II. Assembly
III. Attachment
IV. Release
V....
Which of the following statements comparing virus classification and taxonomy of organisms is true?
Which of the following statements comparing virus classification and taxonomy of organisms is true?
A) Both viruses and organisms are organized into...
Which of the following statements regarding virus taxonomy is true?
Which of the following statements regarding virus taxonomy is true?
A) Viruses are classified on the basis of the taxonomy of their host organisms.
B)...
Which of the following would NOT be found as a component of a bacteriophage?
Which of the following would NOT be found as a component of a bacteriophage?
A) envelope
B) icosahedral capsid
C) DNA
D) tail fibers
E) base plate
Answer:...
Which of the following infectious particles do NOT have protein in their structure?
Which of the following infectious particles do NOT have protein in their structure?
A) bacteriophages
B) prions
C) animal viruses
D) viroids
E) both...
How are fungal viruses different from viruses that infect other organisms?
How are fungal viruses different from viruses that infect other organisms?
A) They have no extracellular state.
B) They have only DNA for genetic...
Who was the first person to demonstrate the existence of viruses?
Who was the first person to demonstrate the existence of viruses?
A) Stanley
B) Rous
C) Lacks
D) Ivanowsky
E) Prusiner
Answer:...
Host specificity of a virus is due to
Host specificity of a virus is due to
A) particular genes that it shares with the infected cell.
B) the presence of an envelope.
C) differences in...
Viruses are primarily classified according to their
Viruses are primarily classified according to their
A) type of nucleic acid.
B) type of life cycle.
C) number of chromosomes.
D) shape.
E) size.
Answer:...
During the intracellular state, a virus exists as
During the intracellular state, a virus exists as
A) a lipid.
B) a protein.
C) a nucleic acid.
D) both a protein and a nucleic acid.
E) both a lipid...
The outermost layer of a virion fulfills which of the following functions of the virus?
The outermost layer of a virion fulfills which of the following functions of the virus?
A) protection
B) recognition
C) replication
D) protection...
Which of the following statements concerning viruses is FALSE?
Which of the following statements concerning viruses is FALSE?
A) Viruses never contain both DNA and RNA.
B) Viruses enter a cell to complete the...
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