Which is the correct procedure for microbiological aseptic technique when working with cultures in test tubes?

Which is the correct procedure for microbiological aseptic technique when working with cultures in test tubes?



A) remove cap, wave test tube over flame, dip inoculating loop, put cap back on
B) remove cap, dip inoculating loop, put cap back on
C) remove cap, wave test tube over flame, flame sterilize loop, dip loop into culture, put cap back on
D) remove cap, wave over flame, flame sterilize loop, dip loop into culture, wave test tube over flame again, put cap back on

Answer: D) remove cap, wave over flame, flame sterilize loop, dip loop into culture, wave test tube over flame again, put cap back on


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