You have isolated a bacterium that you believe to be the causative agent of a new disease in frogs. How would you test the third of Koch's postulates?
a. Determine the shape of the bacterial cells.
b. Inject the bacteria into a healthy frog.
c. Isolate the bacterium from a sick frog.
d. Show that the bacterium is NOT present in healthy frogs.
e. Grow a pure culture of the bacterium outside the frog.
Answer: Inject bacteria into a healthy frog
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