Which of the following techniques will you use to identify unknown virulence genes that are only expressed in the host?

Which of the following techniques will you use to identify unknown virulence genes that are only expressed in the host? 



a. in situ hybridization of cultured bacteria
b. creating genomic library of open reading frames of pathogen
c. mutational studies in vitro
d. culture techniques
e. in vivo expression technology


Answer: in vivo expression technology


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